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한국식물학회 / v.38, no.3, 1995년, pp.259-266 
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( Mutational Analysis of the Region between vir Box and -35 Sequence in virE Promoter of pTiA6 ) |
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| 초 록 |
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| To investigate how the dyad symmetry region (DSR) and the distance between vir box and -35 sequence of the virE promoter plays a role in virE gene expression, two mutants were constructed by base substitution and insertional mutagenesis. The base substitutional mutation, a AAlongrightarrowCG substitution at positions -39 and -40 on the DSR, showed the level of $eta$-galactosidase activity approximately 91% of the wild type virE promoter activity. Therefore, the native structure of the DSR seems to be not essential for virE expression. The insertional mutation, constructed by inserting 8 bp ClaI linker between -49 and -50, displayed the $eta$-galactosidase activity at 12% of the native virE promoter activity. However, this striking reduction appears to be not caused by destruction of the native DSR structure, but by shifting the vir box far from putative -35 sequence. |
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| 키워드 |
| virE promoter;base substitution;insertional mutation; |
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Journal of Plant Biology / v.38, no.3, 1995년, pp.259-266
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199511920117592)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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