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한국식물학회 / v.38, no.3, 1995년, pp.267-274 
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역전사 중합효소련쇄반응(RT-PCR)과 제한효소 분석을 이용한 오이 모자이크 바이러스의 신속한 검정과 동정
( Rapid Detection and Identification of Cucumber Mosaic Virus by Reverse Transcription and Polymerase Chain Reaction (RT-PCR) and Restriction Analysis ) |
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| 초 록 |
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| Based upon the nucleotide sequence of As strain of cucumber mosaic virus (CMV-As0 RNA4, coat protein (CP) gene was selected for the design of oligonucleotide primers of polymerase chain reaction (PCR) for detection and identification of the virus. Reverse transcription and polymerase chain reaction (RT-PCR) was performed with a set of 18-mer CMV CP-specific primers to amplify a 671 bp fragment from crude nucleic acid extracts of virus-infected leaf tissues as well as purified viral RNAs. The minimum concentrations of template viral RNA and crude nucleic acids from infected tobacco tissue required to detect the virus were 1.0 fg and 1:65,536 (w/v), respectively. No PCR product was obtained when potato virus Y-VN RNA or extracts of healthy plants were used as templates in RT-PCR using the same primers. The RT-PCR detected CMV-Y strain as well as CMV-As strain. Restriction analysis of the two individual PCR amplified DNA fragments from CMV-As and CMV-Y strains showed distinct polymorphic patterns. PCR product from CMV-As has a single recognition site for EcoRI and EcoRV, respectively, and the product from CMV-Y has no site for EcoRI or EcoRV but only one site for HindIII. The RT-PCR was able to detect the virus in the tissues of infected pepper, tomato and Chinese cabbage plants. |
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| 키워드 |
| 오이 모자이크 바이러스;외피단백질 유전자;역전사 중합효소연쇄반응;제한효소분석;바이러스 유전자 검정;cucumber mosaic virus(CMV);coat protein geng;RT-PCR;restriction analysis;molecular detection; |
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Journal of Plant Biology / v.38, no.3, 1995년, pp.267-274
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199511920117601)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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