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한국식물학회 / v.38, no.4, 1995년, pp.359-364 
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( Partial Characterization of Soybean cDNA Encoding CTP: Phosphocholine Cytidylyltransferase ) |
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| 초 록 |
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| As the first step to elucidate the relationship between the structure and function of CTP:phosphocholine cytidylyltransferase (EC 2.7.7.15) in plants, the partial nucleotide sequence of soybean cytidylyltransferase cDNA was determined using a polymerase chain reaction (PCR). Degenerate oligonucleotide primers were synthesized from the conserved region revealed from the rat and yeast cytidylyltransferase DNA sequences. The catalytic domain region showed 78 and 76% homology with the rat and yeast amino acid sequences, respectivly. The hydropathy profile indicated that the C-terminal non-catalytic portion of the protein was very hydrophilic, and in the region between the catalytic domain and the C-terminal region, there was a large amphipathic $alpha$-helical domain that was believed to bind the membrane surface in the active formation. There are 7 potential sites for phosphorylation by protein kinase C and 4 potential sites for phosphorylation by Ca2+/calmodulin kinase within the determined sequence. |
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| 키워드 |
| soybean;CTP;phosphochocholine cytidylyltransferase;phosphatidylcholine;amphipathic helix; |
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Journal of Plant Biology / v.38, no.4, 1995년, pp.359-364
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199511920117485)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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