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한국식물학회 / v.29, no.4, 1986년, pp.285-294 
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당근(Daucus carota L.)배양세포의 DNase활성에 미치는 Polyamines의 영향
( The Effect of Polyamines on the DNase Activity in Cultured Carrot Cells ) |
| 윤미정; 연세대학교 이과대학 생물학과;
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| 초 록 |
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| The present study was attempted to investigate the effects of polyamines such as putrescine, spermidine and spermine on protein content and DNase activity in vivo and in vitro in carrot embryos. It was also investigated whether polyamines could replace role of cations required for DNase activity in vitro. The results obtained are as follows. Putrescine, spermidine and spermine increased protein content, although response to spermine reached plateau at the concentration of 0.1 mM. DNase activity was inhibited by polyamines, the inhibition being concentration-dependent and the highest att he concentration of 10 mM. The inhibition of DNase activity was the most prominent with spermine. Similar inhibitory effect to polyamines which was concentration-dependent was found in DNase activity but no change was shown on time-course in vitro. Putrescine and spermidine enhanced the DNase activity at low Mg2+ and Mn2+ concentrations, suggesting that the role of Mg2+ and Mn2+ for DNase activity could be, in part, replaced by these polyamines. These results, therefore, suggest that plyamines can modulate DNase activity through binding to DNA rather than direct effect on DNase activity. |
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| 키워드 |
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Journal of Plant Biology / v.29, no.4, 1986년, pp.285-294
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO198611920113944)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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