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한국식물학회 / v.29, no.4, 1986년, pp.233-242 
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인삼 근절편 배양시 Peroxidase Isozyme에 관한 연구
( Peroxidase Isozyme in Root Differentiation from Cultured Ginseng Root Explants ) |
| 김명원; 연세대학교 문리과대학 생물학과;
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| 초 록 |
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| In order to pursue some physiological studies on organogenesis in ginseng tissue culture, ginseng root explants were cultured on a modified MS medium containing NAA and kinetin. The activities of peroxidase and some enzymes were investigated and their isoenzyme patterns were also observed. The activity of peroxidase decreased by 20% in one week's culture and increased thereafter by 80% in culturing for 7 weeks compared with the control group. Glucose-6-phosphate dehydrogenase activity increased by 400% after culturing for 5 weeks and increased during the days preceeding root formation. The activities of glutamate dehydrogenase and acid phosphatase also increased during the culture. After 3 weeks' culture, new peroxidase isozyme (pH 7.6) appeared and 7 weeks' culture, another new peroxidase isozyme (pH unidentified) appeared. These patterns were also identified by using FPLC. After 7 weeks' culture, a new esterase isozyme of pH 8.5 appeared and isozyme patterns of acid phosphatase were quite changed compared with the isozyme patterns of tissue cultured for 5 weeks. In so far as these new isoenzymes appear distinctively after 7 weeks' culture, root differentiation is supposed to be induced after 7 weeks' culture. |
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| 키워드 |
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Journal of Plant Biology / v.29, no.4, 1986년, pp.233-242
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO198611920113899)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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