|
|
|
한국식물학회 / v.40, no.2, 1997년, pp.89-94 
|
( Effect of Humidity on Somatic Embryogenesis in Cotyledon Explant Culture of Carrot ) |
| ;;; ;;;
|
|
|
 |
|
| |
| 초 록 |
|
|
| In order to clarify the influence of low humidity culture on the structure and function of somatic embryo the cotyledon explants of Daucus carrota L. cv. Hongshim wre cultured in the petridish whose lids had holes sealed with millipore filters. In the low humidity culture, the production of somatic embryos was enhanced and their maturation promoted but the cotyledon structure of somatic embryos were nearly similar to control. In addition, the low humidity culture improved the germination of somatic embryos. Especially, the germination frequency of jar-shaped embryos was much improved (68%) in comparison with that of jar-shaped embryos formed in constant humidity culture (23%). But low humidity culture at its extreme became an obstacle to normal plant regeneration in that precocious embryos were generated and the primary embryos turned into callus and formed secondary embryos. Therefore it is suggested that moderately low humidity culture (80-90% R.H.) is important to the higher production and better-quality of somatic embryos. |
| |
| 키워드 |
| low humidity;embryo production frequency;Daucus carrota; |
| |
|
|
|
|
Journal of Plant Biology / v.40, no.2, 1997년, pp.89-94
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199711920118786)
언어 : 영어 |
|
| 논문 제공 : KISTI 한국과학기술정보연구원 |
|
|
|
|
|
|
