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한국식물학회 / v.40, no.2, 1997년, pp.95-101 
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( Suppression of $Ca^{2+}$-Influx after ACC-Uptake by Spermine in Vigna radiata ) |
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| 초 록 |
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| Exogenous ACC-induced ethylene production in mung bean hypocotyl segments was severely inhibited by the treatment of spermine in a 6 hr incubation. In protoplasts acquired from the same tissues and pretreated with ACC, the concentration of cytosolic Ca2+ was increased after the addition of exogenous Ca2+. However, this increase of Ca2+ concentration was strongly suppressed by spermine. In previous studies, an artifical increase of Ca2+ influx by the treatment of a Ca2+-ionophore stimulated ACC-induced ethylene production. The inhibitory effect of spermine on ACC-induced ethylene production was more prominent than that of putrescine which has fewer NH3+ groups than spermine. In addition, spermine more promintly suppressed ACC-induced ethylene production in protoplasts in which fewer Ca2+ ions were released from Ca2+-storage organelles. Also, the amount oftranscript of ACC-oxidase which converts ACC to ethylene was decreased by the treatemnt of spermine. However, this reduction resulted only through the suppressin of ethylene production in a 2 hr incubation of mung bean segments. On the basis of these results, we suggest that there isa coupling of ACC-uptake to the increase of cytosolic Ca2+ concentration. In addition, the reduction of exogenous ACC-induced ethylene production by spermine could have resulted, at least partially, from reducing the Ca2+ influx which stimulates ACC-oxidase activity. |
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| 키워드 |
| ACC;ACC-oxidase;ethylene;$Ca^{2+}$;spermine; |
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Journal of Plant Biology / v.40, no.2, 1997년, pp.95-101
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199711920118806)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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