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한국식물학회 / v.40, no.2, 1997년, pp.110-114 
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( Transcription of Human Interleukin-2 Gene in Nicotiana tabacum Driven by CaMV35S Promoter ) |
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| 초 록 |
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| Human interleukin-2 (IL-2) gene was inserted next of the 35S-transcript promoter of cauliflower mosaic virus in a binary vector and introduced into tobacco genome by Agrobacterium-mediated transformation. Genomic DNA blot analysis of the in vitro regenerated transgenic tobacco confirmed that the introduced human IL-2 gene was successfully integrated into tobacco genome. RNA blot analyses were carried out for the IL-2 transcript to exaine expression of the introduced IL-2 gene. Specific signals only from the transgenic tobacco plants indicated that the introduced human IL-2 gene was properly transcribed. The transgenic tobacco plants were self-pollinated and the seeds were harvested. Differences in the level of the transcript were more apparent during the germination of the seeds and between and T2 generation tobacco plants. |
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| 키워드 |
| CaMV35S promoter;human IL-2 transcript;Nicotiana tabacum; |
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Journal of Plant Biology / v.40, no.2, 1997년, pp.110-114
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199711920118846)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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