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한국식물학회 / v.41, no.4, 1998년, pp.255-261 
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( Transgenic Tobacco Plants Expressing the Coat Protein of Cucumber Mosaic Virus Show Different Virus Resistance ) |
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| 초 록 |
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| Transgenic tobacco (Nicotiana tabacum cv. Xanthi-nc) plants were regenerated after cocultivation of leaf explants with Agrobacterium tumefaciens strain LBA4404 harboring a plasmid that contained the coat protein (CP) gene of cucumber mosaic virus (CMV-As). PCR and Southern blot analyses revealed that the CMV CP gene was successfully introduced into the genomic DNA of the transgenic tobacco plants. Transgenic plants (CP+) expressing CP were obtained and used for screening the virus: virus-resistant, delay of symptom development, and susceptible type. Most of the CP+ transgenic tobacco plants failed to develop symptoms or showed systemic symptom development delayed for 5 to 42 days as compared to those of nontransgenic control plants after challenged with the same virus. However, some CP+ transgenic plants were highly susceptible after inoculation with the virus. Our results suggest that the CP-mediated viral resistance is readily applicable to CMV disease in other crops. |
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| 키워드 |
| CMV;coat protein;tobacco;transgenic plant;virus resistance; |
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Journal of Plant Biology / v.41, no.4, 1998년, pp.255-261
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199811920119163)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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