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Çѱ¹½Ä¹°ÇÐȸ / v.42, no.1, 1999³â, pp.75-80

( A cDNA Clone from Broccol (Brassica oleracea) for a New Type of Oleosin )
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From a differential screening of a cDNAlibrary for broccoli anther, a cDNA clone for oleosin was isolated and named BASC1. BASC1 did not contain the amino-terminus but probably coded for most of the protein. The open reading frame extending for 212 amino acids had a very long hydrophobic region flanked by amphiphatic regions, the typical pattern for oleosins. In the long hydrophobic region, BASC1 showed strong homologies to the previously reported oleosins. To the carboxy-terminus from the center of ORE, BASC1 is unique in having a region of hydrophilicity in the middle of the amphiphatic stretch. Transcripts of BASC1 were detected from the anther of uninucleate and binucleate pollens, and from the tapetum and pollen.
 
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oleosin;cDNA;broccoli;carboxy-terminus extension;
 
Journal of Plant Biology / v.42, no.1, 1999³â, pp.75-80
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ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199911920120745)
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