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한국식물학회 / v.42, no.3, 1999년, pp.232-238 
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( Water and Nonelectrolyte Permeability of Plant Cell Membranes after Short Term Application of Amino Acids and Phophorylated Amino Acids ) |
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| 초 록 |
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| The effects of amino acids (aa) and N-(diisopropyloxyphosphoryl)-amino acids (DIPP-aa) on cell membranes were investigated by evaluating water and methyl urea permeability. permeability coefficients Pf and Ps were determined by standard osmotic methods for cells of Pisum sativum stem base epidermis after 20 min exposure to a 5mM solution of each aa and DIPP-aa. The Pf value of P. sativum epidermal cells (untreated controls) was 1.3$pm$0.4$ imes$10-3${mu}{ extrm}{m}$s-1. Treat ments with the diisopropyl-oxyphosphoryl derivatives of three one charged and three polar amino acids (serine, threonine, asparagine, and aspartic acid) and unsubstituted (free) serine and threonine increased water permeability up to about two fold of the control value. Serine and threonine and their DIPP-derivatives increased methyl urea permeability (controls 1.03$pm$0.09$ imes$10-3${mu}{ extrm}{m}$s-1) 30 to 80 percent. Other amino acids and their DIPP-derivatives increased the osmotic water and methyl urea permeation through the plasma membrane. The specificity of these molecules on plasma membranes suggests that the active amino acids (serine and threonine) and their DIPP-derivatives interact with charged membrane molecules. The relatively small changes in water and methyl urea permeability may indicate that the effective aa's and their DIPP-derivatives interact with phospholipids rather than aquaporin. A concurring alteratin of water channel proteins, however, cannot excluded. |
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| 키워드 |
| Permeability coefficient;(Plasmamembrane);serine;solute permeability;threonine;water transport; |
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Journal of Plant Biology / v.42, no.3, 1999년, pp.232-238
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199911920120393)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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