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한국식물학회 / v.42, no.4, 1999년, pp.302-309 
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( Potential Target Gene and Protein Interaction of Pimpinella brachycarpa HD-Zip Protein, Phz4 by Yeast Two-Hybrid System ) |
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| 초 록 |
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| The yeast two-hybrid system was used to investigate dimerization between proteins of Phz2 and Phz4 clones of the homeodomain-leucine zipper family which were obtained by screening a Pimpinella brachycarpa shoot-tip cDNA library. Assays showed that Phz4 formed a homo rather than a heterodimer with Phz2. In addition, we isolated cDNA clones, Phyb1, Phyb2, and Phyb3, that encode proteins interacting with Phz4. Although Phyb1 is not a HD-Zip protein, the activity of interaction between Phyb1 and Phz4 was, surprisingly, stronger thn that of the homodimerization of Phz4. The analysis fo interacting parts indicated that from 1 bp to 466 bp of Phyb1, there was no interaction with Phz4, but from 467 bp to 593 bp, interactions were found with the N-terminal and C-terminal regions, except for HD-Zip of Phz4. This region of Phyb1 contained a nuclear localization signal. DNA-binding analysis showed that the Phz4 HD-Zip domain recognized the [T(C/G)ATTG] core sequence and the region containing the [TCATTG] motif, which is, in itself, a promoter in vitro. |
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| 키워드 |
| homeodomain;lucine zipper;protein-protein-proein ineraction;target gene; |
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Journal of Plant Biology / v.42, no.4, 1999년, pp.302-309
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199911920120217)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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