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한국식물학회 / v.43, no.1, 2000년, pp.48-55 
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( Differential Expression of the Potato Proteinase Inhibitor II Promoter in Transgenic Tobacco ) |
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| 초 록 |
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| We studied temporal and spatial expression patterns of the potato proteinase inhibitor II(PI-II) promoter, using transgenic tobacco (Nicotiana tabacum L. cv. Xanthi) plants that carried of fusion between the PI-II promoter and the chloramphenicol acetyltransferase (cat) gene. PI-II promoter activity was low when plants were young, but increased as plants grew. In 8-week-old plants, old leaves showed higher activity than young leaves. At flowering stage (ca. 15 weeks), the overall promoter activity was reduced to a lower level except in the petals. Compared with stems or petioles at the flowering stage, the roots and floral organs showed minimal activity for the PI-II promoter. We used several environmental stimuli to examine the induction of the PI-II promoter in different organs. Promoter induction was effected by wounding or methyl jasmonate in stems, petioles, sepals, and leaves. The induction was highest in leaves, as was sucrose-enhanced wound induction. These results suggest that the PI-II gene is temporally and spatially regulated. We also established a transient assay system in tobacco BY2 suspension cells to elucidate the upstream regulatory region of the PI-II promoter. A field strength of 0.75 kV/cm and 400 $mu$F capacitance were optimal electroporation conditions for our transient assay. |
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| 키워드 |
| chloramphenicol acty;transferase;electroporation;promoter;proteinase inhibitor;transgenic tobacco; |
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Journal of Plant Biology / v.43, no.1, 2000년, pp.48-55
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200011920127517)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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