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한국식물학회 / v.30, no.1, 1987년, pp.69-77 
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담배(Nicotiana tabacum) 세포배양에 의한 Glyphosate-저항성 개체의 재분화
( Regeneration of Glyphosate-Resistant Plant from Tobacco (Nicotiana tabacum) Cell Culture ) |
| 최상봉; 서울대학교 자연과학대학 식물학과;
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| From the single cell cultures of haploid tobacco (Nicotiana tabacum L. cv. NC 2326) glyphosate-resistant plants were regenerated. After treated with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and then inoculated onto the LS medium supplemented with 1 mM glyphosate, the single cells survived formed colonies and calluses in 65 and 95 days after culture, respectively, and then whole plants were regenerated in 0.1 mM glyphosate-containing medium from the selected calluses. There was no difference in fresh weight and shikimate content between the selected and normal haploid calluses. When sprayed with 0.1 mM glyphosate, the shikimate contents in the regenerated and normal plants were 0.659 and 20.816 mol/g fr. wt., while that in other normal plants which were not sprayed was 0.921. In addition, the calluses induced from the regenerated plants grew without showing any retardation when treated with glyphosate. These results indicate that the secelcted calluses and regenerated plants are resistant to glyphosate. |
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Journal of Plant Biology / v.30, no.1, 1987년, pp.69-77
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO198711920114440)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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