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한국식물학회 / v.30, no.2, 1987년, pp.109-116 
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메칠머큐리젤에 분리된 리보핵산의 니트로셀루로스막으로 이동
( Transfer of RNA from Methylmercury-agarose Gel to Nitrocellulose Membrane ) |
| 안정선; 서울대학교 자연과학대학 식물학과;
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| 초 록 |
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| Effects of staining, buffer washing and denaturing agents on the transferrability of RNA fractionated on a methylmercury hydroxide-agarose gel to a nitrocellulose membrane were studied. Ethidium bromide staining and ammonium acetate buffer washing inhibited RNA transfer, while 3% HCHO and 0.5 M NaOH treatments stimulated transfer which was negated in the ammonium acetate buffer. Accordingly, maintenance of primary structure of RNA was proved to be essential for transferring RNA from the methylmercury hydroxideagarose gel to the nitrocellulose membrane. |
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| 키워드 |
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Journal of Plant Biology / v.30, no.2, 1987년, pp.109-116
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO198711920114348)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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