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한국식물학회 / v.30, no.3, 1987년, pp.201-203 
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간편한 고등식물 RNA 분이 방법
( A Simple Procedure for RNA Isolation from Plants and Preservation of Plant Material for RNA Analysis ) |
| ;; 과학기술원 유전공학센타;과학기술원 유전공학센타;
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| 초 록 |
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| Total RNA was isolated from two months old wheat, rice, tobacco and sweet potato. The procedure used was simple and provided pure RNA preparation. Lysis of plant tissue in a buffer with guanidine thiocyanate and CsCl density gradient centrifugation separated RNA from the rest of the cellular components. Subsequent cholroform/1-butanol extraction and ethanol precipitation were necessary to ensure contaminant-free RNA preparation. Storage of the lysed plant tissue in the buffer with guanidine thiocyanate preserved the sample for two months without noticeable RNA degradation. |
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| 키워드 |
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Journal of Plant Biology / v.30, no.3, 1987년, pp.201-203
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO198711920114304)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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