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한국식물학회 / v.31, no.1, 1988년, pp.41-49 
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벼 진탕 배 배양세포로부터 원형질체 분리 및 배양
( Isolation and Culture of Protoplasts Derived from Embryogenic Cell Suspension Culture of Oryza sativa (Rice) ) |
| 황백;김미경;; 전남대학교 자연대학 생물학과;전남대학교 자연대학 생물학과;미국 플로리다대학교 식물학과;
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| Several cultivars of rice were examined for induction of embryogenic callus on a medium containing MS salts, vitamins and 2, 4-D under darkness. Embryogenic callus was obtained from cultivar Cheonma with high ratio and embryo-like structures were formed from the callus on a medium with or without reduced 2, 4-D. Somatic embryoids with a plumule and radicle axis surrounded by a scutellum were observed. These embryoids germinated and produced plantlets in 30 days on the same medium. Protoplasts isolated from an embryogenic cell suspension culture derived from embryogenic callus were cultured either in liquid or in agar medium and protoplast derived cell colonies were obtained in 3-4 weeks. |
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| 키워드 |
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Journal of Plant Biology / v.31, no.1, 1988년, pp.41-49
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO198811920114466)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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