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한국식물학회 / v.31, no.2, 1988년, pp.91-100 
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당근 현탁배양 세포에서 $Ca^{2+}$과 Polyamines가 Cell Wall 합성에 관여하는 $eta-Glucan$ Synthetase II 활성에 미치는 영향
( Effect of $Ca^{2+}$$eta-Glucan$ Synthetase II Related to Cell Wall Synthesis in Carrot Suspension Cultured Cells ) |
| 표병식; 연세대학교 생물학과;
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| The effect of Ca2+ and polyamines on the activity $eta$-glucan synthetase II(GSII) related to cell wall synthesis was studied in carrot suspension cultured cells. The activity of GS II is four times higher than that of $eta$-glucan synthetase I in carrot suspension cultured cells and in vitro expreiment, the activity of GSII was increased in response to increase in concentration of Ca2+ and polyamines. When carrot suspension cultured cells were incubated together with Ca2+ and polyamines, the GSII activity was high at 0.1mM of Ca2+ and 1mM of putrescine. Also, polycationic poly-L-lysine and poly-L-ornithine increased about 50% the GSII activity than that of the control, respectively. These results may imply that Ca2+ and polyamines were related to the enzyme activity as a polycationic nature. In addition, verapamil as the calcium channel blocker and flunarizine as an antagonist of calcium mechanism in cytoplasm decreased GSII activity ramarkably, Ca2+ and calmodulin stimulated GSII activity as Ca2+ of free ion rather than Ca2+ calmodulin complex. The effect of 2,4-D on the GSII activity in culture medium is shown to be low at 0.1mg per liter and GSII activity increased about 30% more than that of the 0.1mg/l at the range of 0.3-1.0mg per litere. Cummulative results suggest that Ca2+ and polyfamines stimulate the cell wall synthesis by means of the enhancement of GSII activity responsible for synthesizing the cell wall components. |
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| 키워드 |
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Journal of Plant Biology / v.31, no.2, 1988년, pp.91-100
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO198811920114657)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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