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한국식물학회 / v.31, no.2, 1988년, pp.121-130 
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연초 원형질체의 생존율과 세포벽 재생에 미치는 식물생장조절물질의 효과
( Effects of Phytohormones on the Viability and Cell Wall Regeneraton of Tobacco Protoplasts ) |
| 김용옥; 건국대학교 이과대학 생물학과;
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| 초 록 |
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| In order to clarify effects of phytohormones on the viability and the cell wall regeneration of protoplasts isolated from Nicotiana tobacum L. var. BY4, protoplasts isolated from mesophyll tissue were cultured on the Murashige-Skoog liquid media supplemented with auxin(2, 4-D, NAA, IAA) and/or cytokinin (kinetin, BAP, 2ip). Viability of protopplasts was higher in the culture medium containing auxin and cytokinin, especially in the combination of 2, 4-D and BAP. The effectual cell wall regeneration of protolasts was observed when theprotoplasts were cultrued on the medium supplemented with auxin alone, especially with IAA. Cell wall regernation started from 2-3 days after culture and was not detected at budding regions. When the protoplasts were cultured on the phytohormone-free medium, the viability of protoplasts dramatically decreased 4 days after culture. |
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| 키워드 |
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Journal of Plant Biology / v.31, no.2, 1988년, pp.121-130
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO198811920114688)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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