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한국식물학회 / v.32, no.2, 1989년, pp.79-87 
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감자로부터 단백질분해효소 억제제 II 유전자의 분리
( Isolation of Proteinase Inhibitor II Genes from Potato ) |
| 이종섭; 서울대학교 자연과학대학 식물학과;
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| 초 록 |
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| Southern hybridization of genomic DNAs with radioactively labeled cDNA of tomato proteinase inhibitor II revealed that proteinase inhibitor II proteins in potato plants are encoded by a family of about 10 related sequences. Screening of potato EcoRI genomic library with the cDNA resulted in isolation of 13 recombinant phage clones which carry 3 different genomic regions. Of these clones, clones 8, 18, and 39 were subjected to restriction mapping and subcloning. Further characterization of the subclones of clones 8, 18 and 39 indicated that two inhibitor II genes are present on a 8.0 kb EcoRI fragment of clone 8, one on 3.3 and 0.8 kb EcoRI fragments of clone 18 and two genes on a 13.5 kb EcoRI fragment of clone 39. |
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| 키워드 |
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Journal of Plant Biology / v.32, no.2, 1989년, pp.79-87
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO198911920114957)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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