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한국식물학회 / v.32, no.4, 1989년, pp.247-253 
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고구마(Ipomoea batatas)의 현탁배양 세포의 원형질체 배양에 의한 캘러스 형성
( Callus Formation from Suspension Culture-Derived Protoplasts of Sweet Potato(Ipomoea batatas) ) |
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| 초 록 |
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| Protoplasts were enzymatically isolated from suspension culture of sweet potato. High yields of single protoplasts were produced from nonembryogenic cell aggregates. However, most protoplasts obtained from embryogenic cell clumps were spontaneously fused during enzyme treatment; a small portion of them remained single. Upon transfer to Murashige and Skoog's(MS) liquid medium supplemented with 0.1 mg/1 6-benzyladenine(BA) and 1 mg/12,4-dichlorophenoxyacetic acid(2,4-D), protoplasts from nonembryogenic cell aggregates sustained cell divisions to form cellus. Upon subculture onto MS media with 0.2 mg/12,4-D or without growth regulators, the callus did not give rise to any organs. On the other hand, first cell division of single protoplasts from embryogenic cell clumps was sporadically observed. |
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| 키워드 |
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Journal of Plant Biology / v.32, no.4, 1989년, pp.247-253
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO198911920114755)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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