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한국식물학회 / v.32, no.4, 1989년, pp.275-283 
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담배 현탁배양 세포로부터 $eta-1$,4-endoglucanase의 정제 및 성질
( Purification and Properties of $eta-1$, 4-endoglucanase from Tobacco Suspension Cultured Cells ) |
| 이영미; 연세대학교 이과대학 생화학과;
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| 초 록 |
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| $eta$-1, 4-endoglucanase (EC 3, 2, 1, 4) was isolated and purified from Nicotiana tabaccum L. Var. Virginia 115 suspensin cultured cells. The molecular weight as estimated by Sephadex G-100 was about 14, 000. The optimum pH for activity was 5.4. The Km value for carboxymethyl cellulose was 0.18 mg/unit and the enzyme was quite resistant to heating. Polyamine did not affect the activity of $eta$-1, 4-endoglucanase in vitro but the activity increased drastically when polyamines were added in the suspension culture medium. It is suggested that increase in $eta$-1, 4-endoglucenase activity due to polyamine might be related to growth of the plant. |
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| 키워드 |
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Journal of Plant Biology / v.32, no.4, 1989년, pp.275-283
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO198911920114784)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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