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한국식물학회 / v.33, no.2, 1990년, pp.119-126 
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해녀콩(Canavalia lineata)의 잎, 뿌리 및 도관액에서 Canavanine의 함량분석
( The Analysis of Canavanine Content in Leaves, Roots, and Xylem Exudate of Canavalia lineata ) |
| 박경순; 서울대학교 자연과학대학 식물학과;
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| 초 록 |
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| The content of canavanine was measured and analyzed in leaves, roots and xylem exudate of Canavalia lneata. In non-nodulated plants, the cotyledons were removed after a week of sowing and the plants were grown for 3 weeks. The quantity of canavanine measured by canavanine specific-PCAF colorimetric assay was 9-10 $mu$mol/g fresh wt. in leaves, 5-6 $mu$mol/g fresh wt. in roots, and 0.3-0.5 $mu$mol/ml in xylem exudate. When free amino acids of leaves, roots, and xylem exudate were analysed by HPLC, the relative proportion of asparagine plus glycine was the highest and canavanine was high secondarily. And the relative proportion of canavanine among total free amino acids was 30-35% in leaves and roots, and 12-13% in xylem exudate. In non-nodulated plants grown for 8 weeks, the canavanine content of each part was similar to that of 3-week-old plants. By the formation of nodules, the canavanine content of leaves, roots, xylem exudate, and nodules decreased apparently. In xylem exduate, the nitrogenous compounds were also analyzed. The relative contents of NO3-, free amino acids, and ureides(allantoin and allantoic acid) were 60-80%, 20-30%, and 5%, respectively. From these results, it can be assumed that canavanine is synthesized in the root of plant and nodulation affects the canavanine content. It is obvious that canavanine is considered one of the reduced-N forms transported via xylem. |
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| 키워드 |
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Journal of Plant Biology / v.33, no.2, 1990년, pp.119-126
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199011920115181)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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