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한국식물학회 / v.33, no.4, 1990년, pp.321-323 
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옥수수 유식물 조직에서 분리한 막 단백질과 Phosphatidylcholine의 재조합
( Reconstitution of Membrane Proteins from Corn Seedlings with Phosphatidylcholine ) |
| 오승은; 연세대학교 생물학과;
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| 초 록 |
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| Membrane proteins isolated from the coleoptile and mesocotyl tissues of corn seedlings were solubilized with Triton X100 and reconstituted with phosphatidylcholine at 2$0^{circ}C$. The proteoliposomes were incubated and proton uptake into the vesicles was measured with a spectrophotometer. Addition of ATP to the reaction mixture was found to result in an active accumulation of proton into the vesicles. These results indicate that the preparation contains tightly bound phosphatidylcholine vesicles with reconstituted H+ -ATPase from the plant cell membranes. |
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| 키워드 |
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Journal of Plant Biology / v.33, no.4, 1990년, pp.321-323
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199011920115435)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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