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한국식물학회 / v.33, no.4, 1990년, pp.217-223 
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시금치 엽록체와 Chlamydomonas로부터 추출한 제한효소 EcoRI 억제물질의 특성과 작용
( Character and Function of Restriction Enzyme, EcoRI Inhibiting Substance Extracted from Spinach Chloroplast and Chlamydomonas ) |
| 황성빈; 연세대학교 생물학과;
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| 초 록 |
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| Restriction enzyme inhibiting substance (REIS) extracted from spinach chloroplast and Chlamydomonas seems not to be proteinaceous, because its inhibiting activity was not lost by heat or trypsin treatment. And it seems not to be lipid or polysacchride, because its inhibiting activity was not lost by lipase or $alpha$-amylase treatment, respectively. In Chlamydomonas, putrescine, spermidine and spermine were present. The amount of putrescine was the smallest and that of spermie was the greatest. But only spermine was contained in REIS and the activity of REIS. It was proportional to the amount of spermine in REIS and it was hindered by Na+ ion. So, the inhibiting activity of REIS seems to be deeply related to spermine contained in REIS. But restriction enzyme inhibiting activity remained to the some extent although salts and spermine were eliminated by dialysis. |
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| 키워드 |
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Journal of Plant Biology / v.33, no.4, 1990년, pp.217-223
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199011920115312)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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