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한국식물학회 / v.33, no.4, 1990년, pp.293-301 
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Chlamydomonas reinhardtii에서 순화한 Endonuclease의 특징과 Polyamine의 영향
( Characterization of and Polyamine Effect on Endonuclease from Zygotes of Chlamydomonas reinhardtii ) |
| 김재윤; 연세대학교 이과대학 생물학과;
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| 초 록 |
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| We have purified and characterized a deoxyribonuclease from zygotes of the eukaryotic green alga, Chlamydomonas reinhardtii and investigated effects of the polyamines, putrescine, spermidine and spermine on the purifed endonuclease-catalyzed cleavage of plasmid DNA. The enzyme has a molecular weight of about 37 kDa as measured by gel filtration and SDS-polyacrylamide gel electrophoresis. There is no requirement for a divalent cation. The activity is sensitive to ionic strength, as NaCl and KCl result in inhibition. The cleavage of plasmid DNA by the purified endonuclease was effectively inhibited by polyamines. The enzyme activity was inhibited more effectively by spermine than by spermidine. The inhibition by putrescine was lower than the other two polyamines. |
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| 키워드 |
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Journal of Plant Biology / v.33, no.4, 1990년, pp.293-301
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199011920115398)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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