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Çѱ¹½Ä¹°ÇÐȸ / v.34, no.1, 1991³â, pp.53-57
ÀÚ¿±À» Á¦°ÅÇÑ ´ëµÎ À¯½Ä¹°¿¡¼­ Polyamine°ú Methylglyoxal bis(guanylhydrazone)°¡ Diamine OxidaseÀÇ È°¼º¿¡ ¹ÌÄ¡´Â ¿µÇâ
( Effects of Polyamines and Methylglyoxal bis(guanylhydrazone) on Activity of Diamine Oxidase in Soybean (Glycine max) Seedlings without Cotyledons )
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´ëµÎÀÇ ÀÚ¿±À» Á¦°ÅÇÑ À¯½Ä¹°¿¡¼­ polyamine°ú methylglyoxal bis(guanylhydrazone)(MGBG)°¡ diamine oxidaseÀÇ È°¼º¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» Á¶»çÇÏ¿© ´ÙÀ½°ú °°Àº °á°ú¸¦ ¾ò¾ú´Ù. $10^{-2};M$ÀÇ putrescine, spermidine°ú spermineÀ» ó¸®ÇÏ¿´À» ¶§¿¡´Â diamine oxidaseÀÇ È°¼ºÀÌ ¾à 80% ¾ïÁ¦µÇ¾úÀ¸³ª, $10^{-6};M$ÀÇ putrescineÀ» ó¸®ÇÏ¿´À» ¶§¿¡´Â È¿¼ÒÀÇ È°¼ºÀÌ Áõ°¡µÇ¾ú´Ù. ÀÌ´Â ±âÁú ƯÀ̼ºÀÌ Å« putrescine¿¡ ÀÇÇØ diamine oxidase°¡ À¯µµµÉ ¼ö ÀÖ´Ù°í »ç·áµÈ´Ù. MGBGÀÇ ³óµµ°¡ Áõ°¡ÇÔ¿¡ µû¶ó putrescine ÇÔ·®ÀÌ Áõ°¡µÇ¾ú´Ù. In vitro¿¡¼­ diamine oxidaseÀÇ È°¼ºÀº $10^{-3};M$ÀÇ MGBG¿¡¼­ ¾à 40% ¾ïÁ¦µÇ¾ú´Ù. In vivo¿¡¼­ diamine oxidaseÀÇ È°¼ºÀº Àú³óµµÀÇ MGBG¿¡¼­ Áõ°¡µÇ¾ú´Âµ¥, ÀÌ´Â MGBG°¡ putrescineÀ¸·ÎºÎÅÍ spermidine Çü¼ºÀ» ¾ïÁ¦ÇÏ¿© ÀÌ·Î ÀÎÇØ ÃàÀûµÈ putrescineÀÌ putrescineÀ¸·ÎºÎÅÍ spermidine Çü¼ºÀ» ¾ïÁ¦ÇÏ¿© ÀÌ·Î ÀÎÇØ ÃàÀûµÈ putrescineÀÌ diamine oxidase¸¦ À¯µµÇÏ´Â °ÍÀ¸·Î »ç·áµÈ´Ù. ¹Ý¸é, °í³óµµÀÇ MGBG¿¡¼­´Â diamine oxidaseÀÇ È°¼ºÀÌ °¨¼ÒµÇ¾ú´Ù. µû¶ó¼­ °í³óµµÀÇ MGBG¿¡ ÀÇÇØ putrescineÀÇ ÇÔ·®ÀÌ Áõ°¡ÇÏ´Â °ÍÀº MGBG°¡ putrescineÀ» ºÐÇØÇÏ´Â diamine oxidaseÀÇ È°¼ºÀ» ¾ïÁ¦Çϴµ¥ ±âÀÎÇÏ´Â °ÍÀ¸·Î »ç·áµÈ´Ù.
The effect of polyamines and methylglyoxal bis(guanylhydrazone)(MGBG) on the activity of diamine oxidase was studied in soybean (Glycine max) seedlings. $10^{-2};M$ of putrescine, spennidine, and spermine inhibited diamine oxidase activity, whereas $10^{-6};M$ putrescine increased enzyme activity. These results suggest that diamine oxidase can be induced by a specific substrate, putrescine. The content of putrescine was increased in response to the increase in concentratioin of MGBG. In vitro, 40% of the diamine oxidase activity was inhibited by $10^{-3};M$ MGBG. In vivo, the diamine oxidase activity was increased by a low concentrration of MGBG. It was suggested that MGBG inhibited the formation of spermidine and that the accumulated putrescine induced diamine oxidase, whereas the diamine oxidase acitivity was inhibited by a high concentration of MGBG. It is suggested that a high cocentration of MGBG increases the putrescine content by inhibiting diamine oxidase activity which is responsible for putrescine degradation.dation.
 
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Journal of Plant Biology / v.34, no.1, 1991³â, pp.53-57
Çѱ¹½Ä¹°ÇÐȸ
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199111920115517)
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