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Çѱ¹½Ä¹°ÇÐȸ / v.34, no.1, 1991³â, pp.25-30
Æ÷Çöó ÀÙÀýÆíÀÇ Áß´Á¿¡¼­ ºÎÁ¤¾Æ Çü¼º¿¡ ¹ÌÄ¡´Â ¾çÀÌ¿ÂÀÇ ¿µÇâ
( Effects of Cations on Adventitious Bud Formation on the Midvein of Leaf Segment in Poplar )
±è¼öÁø; ¿¬¼¼´ëÇб³ ¹®¸®´ëÇÐ »ý¹°Çаú;
 
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Æ÷ÇöóÀÇ ÀÙÀ» ±âº»¹èÁöÀÎ WPM¿¡ BA 0.2 ppm, NAA 0.01 ppmÀ» ÷°¡ÇÏ¿© ¹è¾çÇÏ¸é ±Ø¼ººÐÈ­°¡ ÀϾ¼­ ´ëºÎºÐÀÇ Á¶Á÷¿¡¼­ ±âºÎ Àý´Ü¸é¿¡ ºÎÁ¤¾Æ°¡ Çü¼ºµÇ´Âµ¥, º» ¿¬±¸´Â $Ca^{2+}$°ú ¸î °¡Áö ¾çÀÌ¿ÂÀÌ Æ÷ÇöóÀÇ ±Ø¼ººÐÈ­¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» Á¶»çÇÏ¿´´Ù. WPM³»¿¡ $Ca^{2+}$ÀÇ ³óµµ¸¦ ³ô¿©ÁÖ¸é ÀÙÀýÆí¿¡¼­ ºÐÈ­µÈ ÀüüºÎÁ¤¾ÆÀÇ ¼ö´Â Áõ°¡ÇÏÁö¸¸ ±Ø¼ººÐÈ­´Â ¾ïÁ¦µÇ¾ú°í, EGTA¸¦ ó¸®Çϸé $Ca^{2+}$¿¡ ÀÇÇÑ ±Ø¼ººÐÈ­ÀÇ ¾ïÁ¦°¡ ´Ù¼Ò µÐÈ­µÇ¾ú´Ù. ÇÑÆí $Ca^{2+}$ÀÇ ³óµµ¸¦ ³ô¿©Áָ鼭, $^{14}C-NAA$ÀÇ Èí¼ö ¹× ºÐÆ÷¸¦ °üÂûÇϸé auxinÀÇ Â÷µîºÐÆ÷´Â Å©°Ô ¿µÇâÀ» ¹ÞÁö ¾Ê¾Ò´Ù. $Ca^{2+}$¿¡ ÀÇÇÑ ±Ø¼ººÐÈ­ÀÇ ¾ïÁ¦Çö»óÀº $Ca^{2+}$ »Ó¸¸ ¾Æ´Ï¶ó ¹èÁö³»¿¡ ÷°¡µÇ´Â ´Ù¸¥ ¾çÀÌ¿ÂÀÎ $Mg^{2+}°ú;K^{+}$ÀÇ ³óµµ¸¦ ³ô¿©ÁØ °æ¿ì¿¡µµ ³ªÅ¸³µ´Ù. µû¶ó¼­ Æ÷ÇöóÀÇ ±Ø¼ººÐÈ­¿¡ ¹ÌÄ¡´Â ¾çÀÌ¿ÂÀÇ ¿µÇâÀº Áö±Ý±îÁö ±Ø¼ººÐÈ­ÀÇ Á¶Àý¿äÀÎÀ̶ó ¾Ë·ÁÁø auxin°ú »óÈ£ÀÛ¿ëÇϱ⺸´Ù´Â ¼¼Æ÷¸· ³»¿ÜÀÇ ÀÌ¿ÂºÐÆ÷Â÷¿¡ ÀÇÇÑ´Ù°í »ý°¢µÈ´Ù.
This study was designed to investigate the relationships of cations to the polar regeneration of adventitious buds on midvein segments of poplar leaves. Basal regeneration was disturbed by adding $Ca^{2+}$. When higher concentration of exogeneous $Ca^{2+}$ was added to the WPM (Woody Plant Medium), the more adventitious buds were formed on midvein segments, and polar regeneration was inhibited. Added $Ca^{2+}$ from 0 to 9.70 mM with 0.5 mM of EGT A to WPM decreased the calcium effect. The treatment of $La^{3+$ with 7.05 mM of $Ca^{2+}$ ovecame the effect of $Ca^{2+}$. The cations $Mg^{2+};and;K^{+}$ also caused regeneration on both basal and distal site. However, polar regeneration was maintained in the medium containing $Ca^{2+}$ without $Mg^{2+}$. The above result suggests that exogenous $Ca^{2+}$ or cations inhibit polar regeneration of poplar.poplar.
 
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Journal of Plant Biology / v.34, no.1, 1991³â, pp.25-30
Çѱ¹½Ä¹°ÇÐȸ
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199111920115478)
¾ð¾î : Çѱ¹¾î
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