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한국식물학회 / v.47, no.1, 2004년, pp.27-32 
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( Possible Involvement of $Ca^{2+}$ -Dependent Protein Kinases in Spore Germination of the Fern Osmunda japonica ) |
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| 초 록 |
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| Fern gametophyte is a good model system to investigate signal transduction in plant cells. In this work, we examined whether CDPKs are involved in the mechanisms of spore germination of the fern Osmunda japonica. A protein extract from the spores included four CDPK isoforms with relative molecular weights of 56, 53, 49, and 47 kDa, as detected by immunoblot analysis, and they showed CDPK-like activities, as detected by in-gel protein-kinase assay. It was also found that the inhibitors effective on CDPKs, such as a general protein kinase inhibitor, K252a, and a calmodulin antagonist, W-7, largely suppressed the spore germination, and that many proteins of the spores were phosphorylated in vivo in a calcium dependent manner in the period when the spores require external $Ca^{2+}$ for the germination. Furthermore, we showed that Sr$^{2+}$ and Mn$^{2+}$, which could substitute for $Ca^{2+}$ in the spore germination, were also able to activate the Osmunda CDPKs. From these results, we concluded that CDPKs would participate in the spore germination of O. japonica.aponica. |
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| 키워드 |
| CDPK;fern gametophyte;Osmunda japonica;spore germination; |
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Journal of Plant Biology / v.47, no.1, 2004년, pp.27-32
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200411922994702)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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