|
|
|
한국식물학회 / v.47, no.1, 2004년, pp.1-7 
|
( Studies on the Mobilization of a Maize Transposable Family, Ac/Ds, in Pepper Using In Vivo Transient Assay System ) |
| ;;;;;;;;; ;;;;;;;;;
|
|
|
 |
|
| |
| 초 록 |
|
|
| A maize transposable family, Ac/Ds, has been successfully utilized as a mutagenizing agent not only in monocot but also in dicot. In order to develop insertional mutagenesis system in pepper, the mobility of Ac/Ds has been examined. In this study, the excision of the elements was monitored via transient assay system with protoplasts. Two different systems were developed and compared; one- and two-elements systems. In a one-element system, Ac alone was introduced into cells. As a two-element system, Ac and Ds were cloned into a single vector and were expressed in protoplasts. Our data showed that both Ac and Ds elements were highly mobile in pepper cells. This is the first report suggesting that Ac/Ds mediated gene tagging system could be successfully operated in pepper. |
| |
| 키워드 |
| Ac/Ds;pepper;transient assay; |
| |
|
|
|
|
Journal of Plant Biology / v.47, no.1, 2004년, pp.1-7
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200411922994626)
언어 : 영어 |
|
| 논문 제공 : KISTI 한국과학기술정보연구원 |
|
|
|
|
|
|
