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한국식물학회 / v.15, no.1, 1972년, pp.28-32 
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담배 반수성의 유엽배양에 관한 연구
( Studies on the Culture of Haploid Tobacco Leaf ) |
| 한창열; 원자력청 방사선농학연구소;
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| 초 록 |
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| Young haploid leaf derived from the anthers of tobacco plant was cultuerd and plantlets of various ploidies were obtained. When the leaf was put on the medium supplemented with kinetin as growth regulator, plantlets developed directly from the leaf, and the plants coming out in early stage of culture were all haploid. Plants developing in later stage were mostly haploids with some exception of diploid and aneuploid. Leaves were also cultured on the callus-inducing media supplemented with 2,4-D and kinetiion, and the calluses were sub-cultured for six months. Plants developed from these calluses were mostly aneuploids of various chromosome numbers. In view of the fact that the plants directly developed from the leaf were all haploid, the tissue of the original leaf explant was assumed to be uniform as far as chromosome number was concerned. On the other hand, it seemed that the occurrence of various ploidies in the plants derived from the calluses of same origin was the result of the influence of in vitro culture. Apical meristem tissues and various multicellular bodies were formed in the epidermal and inner mesophyll tissues as well as in the sub-epidermal cells. |
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| 키워드 |
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Journal of Plant Biology / v.15, no.1, 1972년, pp.28-32
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO197211920111826)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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