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한국식물학회 / v.34, no.3, 1991년, pp.185-190 
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배추 칼루스 배양시 NaCl 및 $Na_2SO_$가 Proline 축적과 수분관계 에 미치는 영향
( Effects of NaCl and $Na_2SO_$ on Proline Accumulation and Water Relations through Callus Cultures of Brassica campestris ssp. pekinensis ) |
| 백기엽; 충북대학교 원예학과;
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| Hypocotyl-derived callus cultures of Brassica pekinensis cv. Chungseng were grown on Murashige-Skoog medium containing NaCl, Na2SO4 and mannitol to clarify the effect of salts on callus growth, proline accumulation and water relations. Na2SO4 was more inhibitory than NaCl when survival rate, growth and fresh:dry weight ratios of established callus were measured. Fresh weight in 0.25% NaCl treatment was more than twice as increasable in comparison to the same concentration of Na2SO4 or control. Proline concentration was increased on either salt, attained at highest levels at the 5th subculture, and fluctuated as affected by both salts or mannitol. Concentrations of redeucing sugars were sharply increased after 2 or 3 times of subculture and decreased by increasing subculture. The water and osmotic potential in callus reached a maximum negative value after two weeks in culture, regardless of salt type and mannitol and tugor remained relatively constant on both salt and mannitol treatments. |
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| 키워드 |
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Journal of Plant Biology / v.34, no.3, 1991년, pp.185-190
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199111920115661)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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