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한국식물학회 / v.34, no.4, 1991년, pp.289-296 
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Agrobacterium rhizogenes에 의하여 형질전환된 인삼(Panax ginseng C.A. Meyer)의 모상근 배양에 의한 Saponin 생산
( Production of Saponin by Hairy Root Cultures of Ginseng (Panax ginseng C.A. Meyer) Transformed with Agrobacterium rhizogenes ) |
| 황백;고경민;황경화;황성진;강영희; 전남대학교 생물학과;전남대학교 생물학과;전남대학교 생물학과;전남대학교 생물학과;연세대학교 생물학과;
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| 초 록 |
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| Cultures of hairy root induced from ginseng(Panax C.A. Meyer) transformed with Agrobacterium rhizogenes (strain A4, ATCC 15834) were established and morphologically two different hairy root strains (HB1, HB2) were obtained. To determine the optimum growth rate, the hairy root (HB2) was cultured in various liquid medium supplemented with or without plant growth hormone. The growth rate of hairy root cultured on MS medium was 1.3-3.1 times higher than those cultured on other media, and the optimum sucrose concentration and pH were 3-6%, 5.5-6.5, respectively. Also, the growth rate of hairy root was increased when 0.02 M ammonium nitrate, 1.2 mM potassium phosphate (monobasic) and 0.5 mg/l IBA were supplied to liquid medium. The saponin patterns and contents of hairy root (HB2) were determined by TLC and HPLC. The crude saponin contents were 4.67% and the total saponin contents were 1.0%, on dry weight basis. |
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Journal of Plant Biology / v.34, no.4, 1991년, pp.289-296
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199111920115784)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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