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Çѱ¹½Ä¹°ÇÐȸ / v.35, no.1, 1992³â, pp.17-23
Æ÷Çöó ÀÙÀýÆíÀÇ ºÎÁ¤¾Æ ºÐÈ­¿¡ ¹ÌÄ¡´Â PolyamineÀÇ ¿µÇâ
( Effects of Polyamines on Adventitious Shoot Regeneration from Populus Leaf Segments )
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Polyamine°ú polyamineÀÇ »ýÇÕ¼º ¾ïÁ¦Á¦°¡ Æ÷Çöó(Populus nigra var. $betulifolia{ imes}Populus$ trichocarpa) ÀÙÀýÆíÀÇ ºÎÁ¤¾Æ ºÐÈ­¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» Á¶»çÇÏ¿© ´ÙÀ½°ú °°Àº °á°ú¸¦ ¾ò¾ú´Ù. PolyamineÀÇ »ýÇÕ¼º ¾ïÁ¦ ¹°ÁúÀÎ DFMA, DFMO ¹× DCHA¸¦ ¹èÁö¿¡ °¢°¢ 1mMÀÇ ³óµµ·Î ó¸®ÇÏ¿´À» ¶§ Æ÷Çöó ÀÙÀýÆíÀÇ »ýÁß·® °¨¼Ò¿Í ÇÔ²² ºÎÁ¤¾ÆÀÇ ºÐÈ­À²ÀÌ °¨¼ÒÇÏ¿´°í ºÎÁ¤¾ÆÀÇ ºÐÈ­µÇ´Â ½Ã±âµµ Áö¿¬µÇ¾ú´Ù. ƯÈ÷, ºÎÁ¤¾Æ ºÐÈ­¿¡ ´ëÇÑ ¾ïÁ¦È¿°ú´Â DCHA°¡ °¡Àå °­ÇÏ¿´À¸¸ç ±× ´ÙÀ½ DFMO, DFMAÀÇ ¼ø¼­·Î ³ªÅ¸³µ´Ù. ÇÑÆí, polyamine »ýÇÕ¼º ¾ïÁ¦Á¦¿¡ ÀÇÇÑ ºÎÁ¤¾ÆÀÇ ºÐÈ­ ¾ïÁ¦ Çö»óÀÌ polyamineÀÇ Ã³¸®¿¡ ÀÇÇÏ¿© °æ°¨ ³»Áö´Â »ó¼âµÇ¾ú´Âµ¥ ÀÌ °úÁ¤¿¡¼­ spermidineÀÌ °¡Àå È¿°úÀûÀ¸·Î ÀÛ¿ëÇÏ¿´´Ù. ÀÌ·¯ÇÑ °á°úµé·ÎºÎÅÍ polyamine, ƯÈ÷ spermidineÀº Æ÷Çöó ÀÙÀýÆíÀÇ ºÎÁ¤¾Æ ºÐÈ­½Ã ÇϳªÀÇ ÇʼöÀûÀÎ ¿ä¼Ò·Î ÀÛ¿ëÇÑ´Ù°í »ç·áµÈ´Ù.
Effects of polyamines and their biosynthetic inhibitors on adventitions shoot regeneration of Populus leaf segments were investigated. Polyamine inhibitors such as difluoromethyl arginine (DFMA), difluoromethyl ornithine (DFMO), and dicyclohexylamine (DCHA) decreased the fresh weight of cultured leaf segment and the rate of adventitious shoot regeneration. The inhibitory effects of DCHA were stronger than any other oplyamine inhibitors, and the rest were in the order of DFMO and DFMA. The inhibitory effects of these inhibitors were lessened or disappeared by the addition of polyamines, among which spermidine was the highest in its effect. Therefore, it is suggested that the spermidine may be related to the adventitious shoot regeneration of Populus leaf segments.gments.
 
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Journal of Plant Biology / v.35, no.1, 1992³â, pp.17-23
Çѱ¹½Ä¹°ÇÐȸ
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199211920115878)
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