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Çѱ¹½Ä¹°ÇÐȸ / v.35, no.3, 1992³â, pp.253-257
¸¶´Ã ¸ðÀÚÀÌÅ© ¹ÙÀÌ·¯½º °Ô³ð¿¡ ´ëÇÑ cDNAÀÇ Å¬·Î´×
( Molecular Coning of cDNA for Garlic Mosaic Virus Genome )
ÃÖ¿¬Èñ; ¼­¿ï´ëÇб³ ºÐÀÚ»ý¹°Çаú;
 
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Potyvirus group¿¡ ¼ÓÇÏ´Â °ÍÀ¸·Î ¾Ë·ÁÁø ¸¶´Ã ¸ðÀÚÀÌÅ© ¹ÙÀÌ·¯½º (GMV)°¡ ½Ä¹°¿¡¼­ º´À» À¯µµÇÏ´Â ¸ÞÄ«´ÏÁòÀ» ÀÌÇØÇϱâ À§ÇÏ¿©, ¸¶´Ã¿¡ Á¸ÀçÇÏ´Â GMV¿¡ ´ëÇÑ cDNA cloneÀÎ clone 29-6À» ºÐ¸®ÇÏ¿´´Ù. Northern blot ºÐ¼®¿¡ ÀÇÇØ ÀÌ ¹ÙÀÌ·¯½ºÀÇ genome size´Â ¾à 9 kbÀ̰í, ¶ÇÇÑ clone 29-6Àº GLV genome°ú À¯»ç¼ºÀÌ ¾ø¾úÀ¸¸ç, ¸¶´ÃÀÙÀ¸·ÎºÎÅÍ ºÐ¸®µÈ $poly(A)^{+}$ RNA¿Í °­ÇÏ°Ô hybridizationµÇ¾ú´Ù. ÀÌ·¯ÇÑ »ç½ÇÀº ÀÌ cDNA cloneÀÌ ¸¶´Ã¿¡ °¨¿°ÇÏ¿© ¸ðÀÚÀÌÅ© º´¹ÝÀ» À¯µµÇÏ´Â GMV¿¡ ´ëÇÑ cDNA clone ÁßÀÇ ÇϳªÀÎ °ÍÀ¸·Î »ý°¢µÇ¾ú´Ù. Clone 29-6À» probeÀ¸·Î »ç¿ëÇÏ¿© ¸¶´Ã ¹ÙÀÌ·¯½ºÀÇ cDNA ÀºÇàÀ» Ž»öÇÏ¿© ÀÌ¿Í ÁßøµÇ´Â cloneÀ» ¼±º°ÇÏ¿© ¿°±â¼­¿­À» °áÁ¤ÇÑ °á°ú À̵éÀÇ ¿°±â¼­¿­ÀÌ ÁßøºÎÀ§¿¡¼­ ¼­·Î ÀÏÄ¡ÇÏÁö ¾Ê¾Ò´Âµ¥ ÀÌ´Â ¸¶´ÃÀº ¿©·¯ ÇüÅÂÀÇ GMV¿¡ ÀÇÇØ °¨¿°µÇ¾î ÀÖÀ½À» ¾Ï½ÃÇÑ´Ù.
Potyvirus group is the largest group among plant virus groups and damages severely plant hosts upon infectiQn. In order to investigate the mechanism by which potyviruses induce disease in plants, a cDNA clone 29-6 which is cOIlsidered to be a cDNA clone for garlic mosaic virus (GMV) was isolated. It did not hybridize to garlic latent virus genome, which is one of two major garlic viruses. Northern blot analysis shows that the genome size of garlic mosaic virus was about 9 kb. Clone 29-6 strongly hybridizes to poly(A) RNA isolated from garlic leaves, suggesting that GMV RNA is polyadenylated as other potyviruses. Nucleotide sequence analysis of cDNA clones overlapping with clone 29-6 showed that garlic plants are infected with various strains of garlic mosaic virus which are closely related to each other. other.
 
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Journal of Plant Biology / v.35, no.3, 1992³â, pp.253-257
Çѱ¹½Ä¹°ÇÐȸ
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199211920116139)
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