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Çѱ¹½Ä¹°ÇÐȸ / v.35, no.4, 1992³â, pp.409-414
QuercetinÀÌ º¸¸® ÀÚ¿±ÃÊ¿¡¼­ ¿Á½Å¿¡ ÀÇÇØ À¯µµµÇ´Â ¿¡Æ¿·» »ý¼º¿¡ ¹ÌÄ¡´Â ¿µÇâ
( Effect of Quercetin on Auxin-induced Ethylene Production in Barley Coleoptiles )
ÀÌÁؽÂ; ÀÌÈ­¿©ÀÚ´ëÇб³ ÀÚ¿¬°úÇдëÇÐ »ý¹°Çаú;
 
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Quercetin(3,5,7,3'-4'-OH-flavone)ÀÌ º¸¸®(Hordeum vulgare var. hexastichon ASCHERS.) ÀÚ¿±ÃÊ Á¶Á÷¿¡¼­ ¿Á½ÅÀ¯¹ß ¿¡Æ¿·» »ý¼ºÀ» ÇöÀúÈ÷ ÃËÁø½ÃÄ×´Ù. $3{ imes}10^{-5};M$ÀÇ quercetin¿¡¼­ ¹è¾çÇÑ º¸¸® ÀÚ¿±ÃÊ Á¶Á÷¿¡¼­ ¿Á½Å À¯¹ß ¿¡Æ¿·» »ý¼ºÀº 4½Ã°£ ÀÌÈĺÎÅÍ Áõ°¡µÇ±â ½ÃÀÛÇÏ¿© 8½Ã°£ ÀÌÈÄ¿¡´Â 200%±îÁö Áõ°¡µÇ¾ú´Ù. QuercetinÀº ³»Àç¿Á½ÅÀÎ indol-3-acetic acid(IAA)¿¡ ÀÇÇÑ ¿¡Æ¿·» »ý¼ºÀº ÃËÁøÇϳª ÇÕ¼º ¿Á½ÅÀÎ 2,4-dichlorophenoxyacetic acid(2,4-D)³ª naphthaleneacetic acid(NAA)¿¡ ÀÇÇÑ ¿¡Æ¿·» »ý¼º¿¡´Â ¾Æ¹«·± ¿µÇâÀ» ÁÖÁö ¾Ê¾Ò´Ù. ¶ÇÇÑ, ¿¡Æ¿·» Àü±¸Ã¼ÀÎ 1-aminocyclopropen-1-carboxylic acid(ACC) À¯¹ß ¿¡Æ¿·» »ý¼º¿¡µµ ÃËÁøÈ¿°ú°¡ ¾ø¾ú´Ù. ÀÌ·¯ÇÑ °á°ú´Â ¿¡Æ¿·» »ý¼º¿¡ ´ëÇÑ quercetinÀÇ È¿°ú´Â ACC Àü´Ü°è¿¡ ÀÛ¿ëÇÑ´Ù´Â °ÍÀ» ÀǹÌÇÑ´Ù. QuercetinÀÌ IAA ´ë»ç¿¡ ¾î¶°ÇÑ ¿µÇâÀ» ¹ÌÄ¡´ÂÁö È®ÀÎÇÑ °á°ú Á¶Á÷À» quercetin¿¡ 1½Ã°£ ¹è¾ç½ÃÄ×À» ¶§ Á¶Á÷³» IAA oxidase È¿¼Ò Ȱ¼ºÀÌ 90% °¨¼ÒµÇ¾úÀ¸¸ç, IAA conjugation¿¡´Â º° ¿µÇâÀ» ¹ÌÄ¡Áö ¾Ê¾Ò´Ù. µû¶ó¼­, ¿Á½Å À¯¹ß ¿¡Æ¿·» »ý¼º¿¡ ´ëÇÑ quercetinÀÇ ÃËÁøÀÛ¿ëÀº quercetinÀÌ IAA oxidaseÀÇ È°¼ºÀ» ¾ïÁ¦½ÃÄÑ ³ô¾ÆÁø IAA ¼öÁØÀÌ ¿Á½Å À¯¹ß ¿¡Æ¿·» »ý¼ºÀ» Áõ°¡½ÃŰ´Â °ÍÀ¸·Î »ý°¢µÈ´Ù.
Effect of quercetin, a kind of natural plant flavonoids, on auxin-induced ethylene production in barley coleoptiles was studied. Auxin-induced ethylene production was apparently stimulated by quercetin. This stimulatory effect of quercetin appeared after 4 h of incubation period. Ethylene production was stimulated 200% over the control after 8 h of incubation by $3{ imes}10^{-5};M$ quercetin. The quercetin effect was most prominent at $10^{-4};M$ of IAA. Ethylene production induced by the synthetic auxin, 2,4-D and NAA, was not significantly affected by quercetin. Also ACC-based ethylene production was unaffected by the flavonoid. In an effort to elucidate mechanisms of quercetin action on auxin-induced ethylene production, the effect of quercetin on 1M metabolism was studied. Data obtained from these experiments indicate that quercetin treatment resulted in about 90% inhibition of IAA oxidase activity. IAA ($3{ imes}10^{-5};M$) conjugation was found to be not affected by quercetin. This results suggest that the stimulatory effect of quercetin on auxin-induced ethylene production may be due to the fact that quercetin inhibits 1M oxidase activity, thus increasing the free IAA level.
 
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Journal of Plant Biology / v.35, no.4, 1992³â, pp.409-414
Çѱ¹½Ä¹°ÇÐȸ
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199211920116290)
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