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한국식물학회 / v.36, no.1, 1993년, pp.1-8 
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포플라 잎절편의 부정아 분화와 Polyamine 함량의 연관성
( Correlations Between Polyamine and Adventitious Shoot Regeneration from Populus Leaf Segments ) |
| 김성호; 서남대학교 생물학과;
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| 초 록 |
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| Polyamine contents and the activities of their main biosynthetic enzymes, arginine decarboxylase (ADC) and ornithine decarboxylase(ODC), were investigated in Populus leaf segments during adventitious shoot regeneration with the addition of 1 millimolar polyamine synthesis inhibitors. From the study of polyamine synthesis inhibitors, ODC was found to be the principal route of polyamine biosynthesis in adventitious shoot regeneration of Populus leaf segments. The ADC inhibitor difluoromethyl arginine(DFMA) and ODC inhibitor difluoromethyl ornithine(DFMO) strongly reduced the putrescine content. On the contarary, DCHA, an inhibitor of spermidine synthase, increased it. Spermidine content was decreased with the treatment of each polyamine synthesis inhibitor, but the inhibitory effect of DCHA was stronger than any other polyamine inhibitor. The decreased polyamine level by polyamine synthesis inhibitors was restored with the exogenously applied polyamine. Comparing the polyamine contents with the adventitious shoot regeneration rate, were observed a close correlation between spermidine content and adventitious shoot regeneration of Populus leaf segments. |
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| 키워드 |
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Journal of Plant Biology / v.36, no.1, 1993년, pp.1-8
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199311920116317)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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