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한국식물학회 / v.36, no.3, 1993년, pp.225-231 
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미치광이풀(Scopolia parviflora)의 모상근 배양에 의한 Tropane Alkaloid 생산
( Production of Tropane Alkaloids by Hairy Root Cultures of Scopolia parviflora ) |
| 안준철; 전남대학교 자연과학대학 생물학과;
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| 초 록 |
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| Transformed hairy roots of Scopolia parviflora, producing tropane alkaloids and native to Korea, were obtained following infection of rhizome segments with Agrobacterium rhizogenes A4. Each root tip induced from inoculum sites excised and cultured in MS agar or liquid medium. About seventy of hairy root clones were established. Among them, several fast growing hairy root clones were examined for alkaliod content. Two dimensional TLC analysis showed that the tropane alkaloid pattern of hairy root was more complicated than that in the rhizome of mother plant. On the other hand, some hairy root clones did not produce scopolamine and hyoscyamine. In HPLC analysis, some hairy root clones yield higher levels of scopolamine and hyoscyamine than those of mother plant rhizome which used for infection. Scopolamine and hyoscyamine were identified by comparison of their retention times and of their spectra data with those of authentic compounds. |
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| 키워드 |
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Journal of Plant Biology / v.36, no.3, 1993년, pp.225-231
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199311920116575)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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