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한국식물학회 / v.36, no.3, 1993년, pp.195-201 
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보리 엽록체의 광계 II에서 수은, 구리 및 아연의 저해효과 비교
( Comparison of Toxic Effects of Mercury, Copper and Zinc on Photosystem II of Barley Cholroplasts ) |
| 전현식; 동의대학교 자연과학대학 생물학과;
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| 초 록 |
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| The room temperature fluorescence induction of chloroplasts was utilized as a probe to locate the site of inhibition by mercury, copper and zinc on PS II by mercury. Inhibitory effect of Hg2+ on electron transport activity was notable as compared with Cu2+ and Zn2+. At concentrations of HgCl2 over 50 $mu$M, activities of PS II and whole-chain electron transport decreased more than 70%, while that of PS I decreased about 10~30%. This suggests that PS II is more susceptible to Hg2+ than PS I is. In the presence of diphenylcarbazide (DPC), 50 $mu$M HgCl2 inhibited the reduction of dichlorophenolindophenol (DCPIP) about 50%. Addition of heavy metals induced marked decrease in maximal variable fluorescence/initial fluorescence [(Fv)m/Fo], but no changes in Fo. With various concentrations of heavy metals, changes of chlorophyll a fluorescence emitted by PS II showed gradual decrease in photochemical quenching (qQ), which indicates an increase in reduced state of electron acceptor, QA. Especially, the addition of HgCl2 caused a notable decrease of qQ. In the presence of 50 $mu$M CuCl2, energy-depended quenching (qE) was completely reduced, whereas in the presence of 50 $mu$M CuCl2 and ZnCl2 it was still remained. The above results are discussed on the effects of mercury in relation to water-splitting system and plastoquinone (PQ) shuttle system. |
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| 키워드 |
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Journal of Plant Biology / v.36, no.3, 1993년, pp.195-201
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199311920116539)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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