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한국식물학회 / v.36, no.3, 1993년, pp.259-266 
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TIB로 처리된 시금치의 엽록체에서 산소발생계의 광재활성화
( Photoreactivation of the Oxygen Evolving Center in TIB-treated Chloroplasts of Spinach ) |
| 정화숙; 경북대학교 사범대학 생물교육과;
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| 초 록 |
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| In Tris-iso-butanol (TIB; Tris buffer pH 8.8 and 1% iso-butanol)-treated chloroplasts, oxygen evolving activity was more inhibited than Tris-treated chloroplasts, but restored highly by 2,6-dichlorophenol-indophenol (DCPIP) and photoreactivation. To understand the mechanism of this results of TIB in photosynthetic electron transport, system, oxygen consumption and evolution of PS I and PS II were measured and protein of the chloroplasts was analysed. In Tris- and TIB-treated chloroplasts, oxygen evolving activity was increased according to the light intensity. Under 48 W·m-2 light intensity, the oxygen evolving activity in both chloroplasts were similar but as the light intensity was increased, TIB-treated chloroplasts showed higher activity. Under 240 W·m-2 light intensity, TIB-treated chloroplasts showed about 25% higher oxygen evolving activity than Tris-treated chloroplasts. Oxygen evolving activity was increased after photoreactivation in both Tris-treated and TIB-treated chloroplasts. Addition of NH4Cl increased the activity in both chloroplasts but in TIB-treated chloroplasts the increase was 30% higher than that in Tris-treated chloroplasts. In PS I, oxygen evolving activity was not inhibited by both treatments whereas in PS II, significant difference was observed between two treatments. Addition of Mn2+ and Ca2+ enhanced oxygen evolution in both Tris- and TIB-treated chloroplasts. Though enhancement was higher in TIB-treated chloroplasts. No difference was observed n protein analysis of the two thylakoid membrane. |
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| 키워드 |
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Journal of Plant Biology / v.36, no.3, 1993년, pp.259-266
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199311920116611)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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