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Çѱ¹½Ä¹°ÇÐȸ / v.36, no.4, 1993³â, pp.391-398
´ç±Ù ¹è¾ç¼¼Æ÷¿¡¼­ RGD Peptide°¡ ¿¡Æ¿·» »ý¼º¿¡ ¹ÌÄ¡´Â ¿µÇâ
( Effect of RGD Peptide on Ethylene Production from Cultured Carrot Cells )
ÀÌÁؽÂ; ÀÌÈ­¿©ÀÚ´ëÇб³ ÀÚ¿¬°úÇдëÇÐ »ý¹°Çаú;
 
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½Ä¹°¿¡¼­µµ µ¿¹°¿¡¼­¿Í °°ÀÌ RGD(Arg-Gly-Asp) sequence¸¦ ¸Å°³·Î ¿øÇüÁú¸·°ú extracellular matrix(ECM)°¡ ¿¬°áµÇ¾î ÀÖ´Ù´Â »ç½ÇÀÌ ¾Ë·ÁÁö°í ÀÖÀ¸¹Ç·Î RGD sequence°¡ ½Ä¹°¼¼Æ÷ÀÇ »ýÀå, ºÐÈ­ µîÀÇ ¿©·¯ °¡Áö »ý¸®ÀûÀÎ Çö»ó¿¡ °ü¿©Çϸ®¶ó´Â °¡´É¼ºÀ» »ý°¢ÇÒ ¼ö ÀÖ´Ù. ±×·¡¼­ RGD sequence°¡ ´ç±Ù(Daucus carota L.) ¹è¾ç¼¼Æ÷¿¡¼­ ¿¡Æ¿·» »ý¼º¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» Á¶»çÇÏ¿´´Ù. RGD sequence¸¦ Æ÷ÇÔÇÏ´Â ÇÕ¼º peptide´Â ´ç±Ù ¹è¾ç¼¼Æ÷¿¡¼­ ¿¡Æ¿·»ÀÇ »ý¼ºÀ» ÃËÁø½ÃÄ×´Ù. RGD peptide´Â Indole-3-acetic acid(IAA)¿Í ÇÔ²² ó¸®ÇÑ °æ¿ì ¿¡Æ¿·» »ý¼ºÀ» ´õ¿í ÃËÁø½ÃÄ×À¸¸ç, ACC synthaseÀÇ È°¼ºµµ Áõ°¡½ÃÄ×´Ù. RGD sequence°¡ ¿¡Æ¿·» »ý¼ºÀ» ÃËÁø½Ãų ¶§ ¾î¶°ÇÑ Æ¯À̼ºÀÌ Àִ°¡¸¦ È®ÀÎÇϱâ À§ÇØ ÀÌ¿Í À¯»çÇÑ RGE(Arg-Gly-Glu) peptide, RGK(Arg-Gly-Lys) peptide¸¦ °¢°¢ ó¸®ÇÏ¿© º¸¾Ò´Ù. RGE peptide´Â RGD peptide¿Í ¸¶Âù°¡Áö·Î ¿¡Æ¿·» »ý¼ºÀ» ÃËÁø½ÃÄ×À¸³ª RGK peptide´Â ¿¡Æ¿·» »ý¼º¿¡ ¿µÇâÀ» ¹ÌÄ¡Áö ¾Ê¾Ò´Ù. µû¶ó¼­ RGD peptideÀÇ ¿¡Æ¿·» »ý¼º ÃËÁøÀÛ¿ëÀº RGD dequence¸¦ Æ÷ÇÔÇÏ´Â RGD peptide°¡ ACC synthaseÀÇ È°¼ºÀ» Áõ°¡½ÃÄѼ­ ÀϾ´Â Çö»óÀ̶ó »ý°¢µÇ¸ç, ¶ÇÇÑ Æ¯Á¤ÇÑ ¾Æ¹Ì³ë»ê ¼­¿­À» ¿ä±¸Çϴ ƯÀÌÀû ¹ÝÀÀÀ̶ó°í »ý°¢µÈ´Ù.
It has been inferred that membrane-ECM (extracellular matrix) interaction in plants may be also mediated by an RGD-dependent recognition system as in animal cells. Effects of RGD peptide on ethylene production was examined in suspension cultured carrot cells. Treatment of the cells with RGD peptide containing RGD (Arg-Gly-Asp) sequence stimulated ethylene production. When RGD peptide was applied to carrot cells treated with 1M, the effect of RGD peptide appeared to be additive. ACC synthase activity in cells pretreated with RGD peptide likewise increased over the control. In an effort to check the sequence specificity of the RGD peptide, cells were treated with substituted RGD peptide, i.e. RGK (Arg-Gly-Lys) and RGE (Arg-Gly-Glu) peptide, respectively. RGK peptide did not stimulate ethylene production but RGE peptide did. The results strongly suggest that the stimulatory effect of RGD peptides on ethylene production may be associated with a physiological phenomenon through a specific recognition between RGD peptide including RGD sequence and their putative plasma membrane receptors.eptors.
 
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Journal of Plant Biology / v.36, no.4, 1993³â, pp.391-398
Çѱ¹½Ä¹°ÇÐȸ
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199311920116794)
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