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한국식물학회 / v.25, no.4, 1982년, pp.181-188 
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콩 원형질체내로의 담배 엽록체 이입
( Incorporation of Tobacco Chloroplasts into Soybean Protoplasts ) |
| 차현철; 서울대학교 자연과학대학 식물학과;
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| 초 록 |
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| Chloroplasts isolated from tobacco (Nicotiana tabacum L. cv. Virginia 115) leaves have been transferred into protoplasts of soybean (Glycine max Merr. cv. Jangyeop) suspension-cultured cells with the help of polyethylene glycol (PEG). The increased yield in protoplasts of chloroplast uptake was depended upon the concentration of both PEG 4,000 and PEG 6,000. The highest yield(36%) occurred at 50% of both PEG, and the yield was decreased above this concentration. The rate of uptake with the incubation time was highest at one hour, then decreased. The process of the chloroplast uptake into the protoplasts was similar with that of a protoplast fusion, except forming invagination during uptake. |
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Journal of Plant Biology / v.25, no.4, 1982년, pp.181-188
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO198211920113550)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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