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한국식물학회 / v.26, no.4, 1983년, pp.191-196 
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강남콩(Phaseolus vulgaris L.) Callus의 원형질체 유이 및 배양
( Isolation and Culture of Phaseolus vulgaris L. Callus Protoplasts ) |
| 김상구; 서울대학교 자연과학대학 식물학과;
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| The isolatin and culture of protoplasts from hypocotyl originated callus of Phaseolus vulgaris cv. Damyang were carried out. The maximum protoplast yield of 4.6$ imes$105 per gram fresh callus, using the 13-day-old callus, was obtained by digeston for 6 hours in the enzyme solution. After 10 day-culture of the isolated callus protoplsts, plating efficiency was 50%. Thereafter, cell cluster medium, and followed by leading to callus formation on an agar medium after 3 weeks of the liquid culture. |
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| 키워드 |
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Journal of Plant Biology / v.26, no.4, 1983년, pp.191-196
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO198311920113593)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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