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한국식물학회 / v.27, no.3, 1984년, pp.139-147 
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Petunia 조직배양시 몇가지 공해물질이 생장반응에 미치는 영향
( Growth Responses of Petunia as Affected by Several Pollutants in Vitro ) |
| 백기엽;최성렬;이재욱;최주견; 충북대학교 이과대학 원예학과;충북대학교 이과대학 원예학과;원예시험장;중앙종묘;
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| 초 록 |
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| This in vitro study was employed to clarify the effects of several pollutants i.e. $SO_2$, fluoride, cadmium(Cd), aluminum(Al) and NaCl, on the organogenesis and growth responses of shoot-tip, stem and multiple-buds segments derived from hypocotyl or cotyledon culture of petunia seedlings. ${Na_2}{SO_3}$levels of more than 200$mu{g}$/ml had significantly reduced organogenesis, growth, and chlorophyll content. The injuries caused by ${Na_2}{SO_3}$, concentration of more than 400$mu{g}$/ml were alleviated by increasing hydrogenion concentration of medium, indicating some relationship between two factors. Organogenesis was not affected by the fluoride concentration up to 100ppm in the media, but the growth and chlorophyll content were greatly reduced by the fluoride. The effect of Cd depended on the explant sources used for the culture; 1.0ppm was effective for fresh weight increase in shoot tip culture, and 3.0ppm in stem segments culture. Organogenesis and growth were greatly reduced by more than 10.0 Cd treatment. Growth and formation of shoots were better with Na conc. of 0.3% compared to control, but those of roots were inhibited. Na concentration goes over 1.0%, organogenesis and subsequent growth were inhibited, and chlorophyll synthesis was drastically reduced. Chlorophyll content was increased on the medium supplemented with Al 50$mu{g}$/ml compared to control. However the formation and growth of shoots were greatly inhibited with more than 400$mu{g}$/ml and roots were not produced at all. |
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| 키워드 |
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Journal of Plant Biology / v.27, no.3, 1984년, pp.139-147
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO198411920127355)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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