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한국식물학회 / v.28, no.3, 1985년, pp.233-241 
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대두 (Glycine max) 부배유 유래 칼루스의 원형질체 분리 및 배양
( Isolation and Culture of Protoplasts from Hypocotyl-derived Callus of Soybean (Glycine max) ) |
| 이광웅; 서울대학교 자연과학대학 식물학과;
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| The isolation and culture of protoplasts from hypocotyl-derived calluses of Glycine max (L.) Merr. cv. Jangyeop were obtained by digestion for 6 hrs in an enzyme solution containing 3.5% cellulase, 1.5% macerozyme, 10% sorbitol and 0.1% CaCl2.2H2O at pH 5.8. Newly formed cell wall of protoplasts cultured in MS agar medium containing 10 $mu$M $alpha$-naphthaleneacetic acid (NAA) and 32 $mu$M N6-benzylaminopurine (BAP) could be observed after 24 hrs culture. The first cell division of the protoplasts was observed after 3 days of culture; cell clusters after 2 weeks of culture. When transferred to solid media, the protoplasts formed cell clusters gave rise to proliferating calluses. |
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Journal of Plant Biology / v.28, no.3, 1985년, pp.233-241
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO198511920113869)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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