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한국식물학회 / v.29, no.1, 1986년, pp.11-18 
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감자(Solanum tuberosum L.) 괴경의 배양세포로부터 원형질체의 분리
( Isolation of Protoplasts from Cultured Cells of Potato (Solanum tubersoum L.) Tuber Tissue ) |
| 정상호; 고려대학교 이과대학 생물학과;
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| 초 록 |
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| Protopasts were isolated from cultured cells of potato (Solanum tuberosum L.) tuber tissue. The ability of callus formation from the culture cells was higher in cultivars Dejima and Superior than in Shimabara and Irish Cobbler on Lam's medium. Therefore, the former was used as sources for protoplast isolation. Friable calli were transferred to liquid media and cells in exponential phase were used for protoplast isolation. In both of Dejima and Superior, the yield of protoplasts was high in the enzyme solution of 2% Onozuka cellulase and 1% macerozyme. Also, viability of isolated protoplasts was very good. Thus, it seems that these protoplasts would be applicable to various aims of research. |
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| 키워드 |
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Journal of Plant Biology / v.29, no.1, 1986년, pp.11-18
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO198611920114153)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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