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Çѱ¹½Ä¹°ÇÐȸ / v.43, no.2, 2000³â, pp.94-98

( The Effects of Spaceflight on Soluble Protein, Isoperoxidase, and Genomic DNA in Ural Licorice (Glycyrrhiza uralensis Fisch.) )
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We investigated the effects of weightlessness, and ionizing radiation plus weightlessness, on changes in the levels of soluble protein, isoperoxidase, and genomic DNA, respectiveIy, in a medicinal plant-ural licorice (Glycyrrhiza uralensis)-after a 15-d spaceflight in a recoverable satellite. Both the weightlessness samples (Ws) and the ionizing radiation plus weightlessness samples (IR/Ws) showed increases in soluble protein content or peroxidase activity, compared with the ground control (Gc). Moreover, the increased isoperoxidase activity for the IR/Ws group was significantly greater than for the Ws, compared with the controls. Likewise, distinctive RAPD profiles were generated among the Ws, the IR/Ws, and the Gc. The Ws and IR/Ws yielded 66 and 78 polymorphic RAPD fragments, respectiveIy, based on bulk template DNA, along with 19 selected primers. Therefore, weightlessness alone can trigger genomic alterations, to some extent, and may even result in modulation of gene expression, whereas ionizing radiation would probably enhance the effort of weightlessness.
 
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genomic alteration;ionizing radiation;RAPD fingerprinting;recoverable satellite;weightlessness;
 
Journal of Plant Biology / v.43, no.2, 2000³â, pp.94-98
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ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200011921106562)
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