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한국식물학회 / v.43, no.2, 2000년, pp.99-106 
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( Identification and Purification of Trans-Acting Factors Binding to the rbcL R2 Promoter Region in Maize (Zea mays) ) |
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| 초 록 |
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| We reported previously that chloroplast DNA binding factors bind to the rbcL promoter R2 (-33 to 229 from ATG) region in Zea mays. In this study, we investigated transcriptional activity in the high salt extracts of chloroplast, and identified rbcL R2 region-specific binding proteins in high-salt extracts. In-vitro transcription and dot blot assays revealed that the extract was transcriptionally active. An electrophoretic mobility shift assay (EMSA) showed that R2 region Binding Factors 1 and 2 (named RBF-1,2) bound specifically to the R2 region. RBF-1 and 2 were precipitated in 0 to 34% and 34 to 51% ammonium sulfate fraction, respectively; their binding activities were detected in 0.2 M and 0.4 M KCI fractions of DEAE cellulose chromatography, respectively. RBF-1 and RBF-2 were purified to about 140- and 66-fold from the chloroplast high-salt extract, respectively, and had specific binding activity to the R2 region. These factors may play an important role in regulating the transcription of rbcL. |
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| 키워드 |
| high-salt extract;rbcL;RBF-1;RBF-2;regulation of transcription; |
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Journal of Plant Biology / v.43, no.2, 2000년, pp.99-106
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200011921106571)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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