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한국식물학회 / v.43, no.3, 2000년, pp.115-120 
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( Relationship between Auxin-Induced Cell Proliferation and Somatic Embryogenesis in Culture of Carrot Cotyledons ) |
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| 초 록 |
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| We elucidated the relationship between cell proliferation and somatic embryogenesis in the culture of carrot cotyledons. Fresh weights of the cotyledon explants were determined every five days while being cultured on a medium containing 2,4-D. Callus produrtion increased exponentially from Day 20 to Day 25, showing a two-fold rate of proliferation. Tn examine the embryogenic potential of the callus, we pre-cultured cotyledon explants on an MS medium with 2,4-D, then transferred them to an MS basal medium at five-day intervals. Somatic embryos formed most frequently when the cotyledons were pre-cultured for 20 days on an MS medium that contained 5 $mu$M 2,4-D. The frequency of somatic embryo formation was 81%, while that of normal embryos with two cotyledons was 51% among those formed on a hormone-free medium. We used FACScan analysis to relate the embryogenic potential of the callus to the S phase in the cell cycle of cultured cells. The S phase was high after 25 days of culture on the medium with 5 $mu$M 2,4-D. In contrast, the frequency of normal embryogenesis was higher at Day 20 of the pre-culture period. Culturing embryogenic calli on a medium with 5 $mu$M 2,4-D was most favorable for producing somatic embryos with two cotyledons. We verified that active somatic embryogenesis was apparently related to tell division activity; somatic embryos induced from actively dividing tells were apt to accompany cotyledonary abnormality, |
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| 키워드 |
| anamalous somatic embryogenesis;cell division;2,4-D;Daucus carota L; |
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Journal of Plant Biology / v.43, no.3, 2000년, pp.115-120
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200011921106603)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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