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Çѱ¹½Ä¹°ÇÐȸ / v.43, no.3, 2000³â, pp.179-182
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( Antibiotics Resistance of a Red Alga, Griffithsia japonica ) |
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| To identify antibiotics suitable for stable transformation, we tested the resistance of a red a18a, Griffithsia japonica Okamura, to four commonly used antibiotirs. Very young germlings, with 1-3 cells, that germinated from the tetraspores were cultured for 40 d in a half PES medium containing kanamycin, streptomycin, hygromycin B, or phleomycin. G. japonica was highly sensitive to 1 $mu extrm{g}$ mL$^{-1}$ of phleomycin and 50 $mu extrm{g}$ mL$^{-1}$ of hygromycin B. However, it was resistant to kanamycin and low levels of streptomycin and hygromycin B. These results suggest that resistance genes for phleomycin or hygromycin B can be used as selectable markers for transformation of C, japonica. |
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| antibiotics;Griffithsia japonica;red alga;transformation; |
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Journal of Plant Biology / v.43, no.3, 2000³â, pp.179-182
Çѱ¹½Ä¹°ÇÐȸ
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200011921106693)
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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